SpectraMax Quant dsDNA Assay Kits. 4 / 5. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. More Product Information. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 蛍光 発光 ELISA. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Explore a wealth of applications with the future-ready SpectraMax i3x. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. D. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Patented user-exchangeable cartridge design expands the system's detection capability making it. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. i3. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Molecular Devicesの全商品を見る. TUNE Tunable Wavelength Detection Cartridge. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. Prime function 20 amol. Microplate readers, multi-mode, SpectraMax® i3x. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. It allows you to explore cellular pathways and protein activation and expression in one system. Contact our support team with your questions regarding Molecular Devices products and applications. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The protocols are also available on the SoftMax Pro Software protocol sharing. com. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 7°C (gradient heating design). HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). ; The top 3 importers of Spectramax are India with 365. Find regional support. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. , 2021). i3. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Amplitude : 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Capture Flash Assays with Ease. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. Published Dec 16, 2020 | Updated Dec 16, 2020. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Examples: fiPress carefully to engage,fl fiDo not press down. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. 22 μ m fi ltration unit (Sigma, cat. 価格、見積もり、お近くの販売店を. applications, including dual luciferase and ATP assays. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. D 1 1. 13. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. *On the SpectraMax i3x reader only. Multimode Reader Comparison Chart. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. 1321N1, a human astrocytoma cell line. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. 313 reference gain check fail. As. It allows you to explore cellular pathways and protein activation and expression in one system. Bioz Stars score: 98/100, based on 1 PubMed citations. Microplate Readers SpectraMax. HTRF IP standard curve. 2C). Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Description 1. Overview of SpectraMax MiniMax 300 Imaging Cytometer. Molecular Devices Software. In addition, Image J was used to quantify the. o For example: Ex: 485/Em 525/Cutoff 515 4. These robust readers have been placed in labs from Antarctica to the. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Description 1. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. 660-665 Eskdale Road. Fluorescence Microplate Reader Comparison Chart. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Explore a wealth of applications with the future-ready SpectraMax i3x. Manufacturers try to design their instruments so they can be. 2 x 10-14. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. SpectraMaxi3Multi. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Increased fluorescence performance. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. edu) to be added as a trained user in iLab. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. eu@moldev. Shake intensity. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. SpectraMax i3x. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Description 1. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. All test measurements and calculations are handlediD5. 312 gain calibration failed. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Open the catalog to page 1. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. pathways and protein activation and expression in one system. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. ZERO BIAS - scores, article reviews, protocol conditions and more. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. StainFree Cell Detection Algorithm. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 흡광 (Absorbance) 리더기. D 1 1. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. 발광 (Luminescence) 리더기. SpectraMax i3x Multi-Mode Detection Platform. *On the SpectraMax i3x reader only. The CellTiter-Glo assay from Promega uses luciferase. 2 (Molecular Devices, San Jose, CA). • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax i3x reader provides the. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Winnersh Triangle. For wavelengths in the UV range above 220 nm,. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. , 1997). Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 1. Fluorescent Polarization: Solid black plates, as above. This is not due to a temperature-dependent alteration in cellular autofluorescence,. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0´ 104 1. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. AquaMax Microplate Washer. Unlock your i3/i3x instrument after shipment. i3x. We would like to show you a description here but the site won’t allow us. The size and fluorescence intensity were tested for 48 h. Increased fluorescence performance. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. A: percent GFP-positive cells calculated using the classification feature in the software. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. 3) At the bottom of the strip, click on "Show. Description 1. , 2011). Nuclear Receptor. iD5. Register to download your free eBook today. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 6 in. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Prime function 20 amol. 제품. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Thetypeof luciferase(Renilla. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. The SoftMax Pro Software provides integrated instrument control. . Generate more data points without the need to dilute. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. ) deep, preferably with access on all sides. 303 unable to cal dark current. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 4 mg mL −1; 1. Prime function 20 amol. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Materials and methodsWe would like to show you a description here but the site won’t allow us. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. *On the SpectraMax i3x reader only. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. Introduction. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Figure 1. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). 635. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 0 mm hex key. • Removing the transport locks from the PMT shutters and drawers. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The SpectraMax i3x reader provides theWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Supplier: Molecular Devices. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. 5. ). The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. com. ATP (“flash” luminescence using . 1. HTRF IP standard curve. 0. Example: [Drawer]. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Thetypeof. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Bioz Stars score: 98/100, based on 1 PubMed citations. 304 signal level saturation. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax ® i3x reader provides the. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Introduction. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. . With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. We would like to show you a description here but the site won’t allow us. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 2 in. , Fisher Scienti fi c, cat. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Microplate Stacker. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 800. 11. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Multimode Reader Comparison Chart. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Louis, MO) and used as received without further purification. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Detection of pro-inflammatory factors. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Nucleic Acid and Protein Quantitation. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Anne T. Order Now. See Unpacking the SpectraMax Paradigm Instrument on page 1. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The drawing shows a standard microplate of 15 mm height covered with a lid. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. ZERO BIAS - scores, article reviews, protocol conditions and more. Methods. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax i3x, onboard luminescence 121. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Ease of use. It allows you to explore cellular pathways and protein activation and expression in one system. The dynamic range of the assay is about 3. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Then, the cell area was calculated for each. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Increased fluorescence performance. Note: Additional readers with similar performance are listed. The information below applies to a standard 96 well plate, set to a linear shake mode. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 5 µL anti-cAMP-Cryptate. As. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. Settings not required by a reader are indicated by ‘---’. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Each heating element is independently. 2 (Molecular Devices, San Jose, CA, USA). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The SpectraMax i2X is used for cell migration, cell. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Pick the best speed, sensitivity, and price for your needs. Italic and boldface type are used for emphasis. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. Find regional support. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The plate was shaken and incubated in the dark for 10 minutes. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 315 can't find zero order. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. ATP (“flash” luminescence using . HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 3: The cuvette chamber. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 200-800 nm using Spectra Max i3X microplate reader. Spectramax I3x, supplied by Danaher Inc, used in various techniques. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Fluorescence Microplate Reader Comparison Chart. Videos & Webinars.